Fedorov Aleksandr Anatolevich (RU),Федоров Александр Анатольевич (RU),Dyu Feliks Chimenovich (RU),Дю Феликс Чименович (RU),Lyublinskaya Irina Nikolaevna (RU),Люблинская Ирина Николаевна (RU),Lyublinsk
申请号：
RU2019118567
公开号：
RU0002700444C1
申请日:
2019.06.14
申请国别(地区):
RU
年份:
2019
代理人:
摘要：
FIELD: biochemistry.SUBSTANCE: invention relates to production of iodized whey proteins for preparing a biologically active substance and can be used for preventing iodine deficiency disorders in humans and animals. Process of iodination of initial protein raw material, in form of α-lactalbumin, β-lactoglobulin or a mixture of said proteins, or hydrolysates of said proteins, by mixing with an aqueous solution of inorganic iodine at temperature of 20–40 °C and at ratio of solution of inorganic iodine to total protein selected (2–40):1. Mixture of whey proteins is fermented with an aqueous solution of inorganic iodine by adding a buffer mixture of reagents – a complex of NaCl mineral salts and Na and K phosphates and a mixture of lactoperoxidase-based enzymes containing 16–24 wt%, horseradish peroxidase and from 14 to 21 wt% of catalase. Buffer mixture contains 14–18 wt% sodium phosphate and 22–28 wt% potassium orthophosphate with stability pH=6–8 with a reaction mixture of enzymes immobilized on semi-permeable membranes or on inert carriers. Fermentation process is carried out with continuous monitoring of iodine content in the solution. Aqueous solution of iodinated proteins is purified from macroimpurities and microimpurities, including from inorganic iodine, using macrofiltration, microfiltration with subsequent diafiltration of aqueous solution of iodinated proteins on ultrafiltration unit in tangential flow mode using membrane modules with cut-off limit of 300 to 800 Da at pH 6.0–8.0. Obtained solution of iodated proteins subject to sterilizing microfiltration, then sublimation or spray drying to obtain a finished powder product with content of deterministic covalently bound iodine in amount of 0.5–4 % in form of a mixture of iodised amino acids – monoiodotyrosines in amount of 55–75 wt% in iodated proteins, 24.0–43.5 wt% di-iodotyrosines and 1.0–1.5 wt% of tri-iodotyrosines.EFFECT: invention enables to obtain a ready powder product with given content of determi
