PROBLEM TO BE SOLVED: To provide an effective sanitizer usable in sterilization of microbes sticking to raw materials, washing of secondarily contaminating microbes, sterilization of microbes sticking on production machine tools and sterilization of microbes in the environment of food-processing industries, facilities for providing meals, or the like, and washing and disinfection of hands and fingers of operators as a countermeasure for controlling microbes in meat and processing industries. SOLUTION: The sanitizer is prepared by dissolving a glycerol fatty acid ester in ethanol, combining the resultant solution with a basic amino acid, a basic protein, an amino acid and an organic acid, an organic acid salt, or the like, mixing and stirring the resultant mixture and the sanitizer contains≤65 vol.% of alcohol. COPYRIGHT: (C)2004,JPO