HEAT SHOCK PROTEIN 70 PROMOTER FOR SILKWORM TRANSFORMATION, PRIMERS FOR AMPLIFICATION THEROF, EXPRESSION VECTOR COMPRISING PROMOTER, SILKWORM TRANSFORMANT TRANSFORMED WITH EXPRESSION VECTOR AND PRODUCTION METHOD THEROF
PURPOSE: A HSP70 promoter for transforming silkworm, a primer for amplifying the same, and an expression vector containing the promoter are provided to express green fluorescent genes in silkworm eggs and larvae, and to early determine transformation. CONSTITUTION: A HSP70 promoter for transforming silkworm is denoted by sequence number 1, and is isolated in a silkworm. A primer for amplifying the promoter has sequences of sequence numbers 2 and 3. An expression vector for transforming silkworm contains the HSP70 promoter. A method for preparing a silkworm transformant comprises: a step of treating a dormant silkworm egg with hydrochloric acid at 25 deg. C for 1 hour; a step of transducing green fluorescent gene at downstream of the HSP70 promoter(sequence number 1) and cloning fused vector to prepare the expression vector; and a step of microinjecting the expression vector to the silkworm egg.
