BioNTech RNA Pharmaceuticals GmbH;TRON - Translationale Onkologie an der Universitätsmedizin der Johannes Gutenberg-Universität Mainz gemeinnützige GmbH
发明人:
Beissert, Tim,Sahin, Ugur,Perkovic, Mario
申请号：
AU2017236240
公开号：
AU2017236240A1
申请日:
2017.03.13
申请国别(地区):
AU
年份:
2018
代理人:
摘要：
The present invention generally relates to systems and methods suitable for high-level protein production. While one or more elements of the present invention are derived from an alphavirus, the present invention does not require propagation of virus particles. In particular, a system comprising two separate RNA molecules is foreseen, each comprising a nucleotide sequence derived from an alphavirus: one RNA molecule comprises a RNA construct for expressing alphavirus replicase, and one RNA molecule comprises a RNA re pi icon that can be replicated by the replicase in trans. The RNA construct for expressing alphavirus replicase comprises a 5'-cap. It was surprisingly found that the 5'-cap is suitable for efficiently driving expression of a transgene from the replicon
