This invention designates that production manner of the amino acid high content yeast which contains separation amino acid in high concentration is offered as purpose. As for this invention, the yeast which is stationary phase of breeding, production manner of the amino acid high content yeast which features that the liquid it cultures under the condition pH of liquid nutrient medium being under the 11 of 7.5 or more, pH of liquid nutrient medium of yeast which is in stationary phase of breeding under the 11 of 7.5 or more after the adjusting, furthermore production manner of the amino acid high content yeast which features that it cultures, the aforementioned yeast satsukaromaisesu serebishie (Saccharomyces cerevisiae), Candidums yuteirisu (Candida utilis) in production manner of the amino acid high content yeast which is and production manner of the aforementioned no amino acid high content yeastDepending, the amino acid high content yeast which can, separation amino acid content, per dry yeast fungus body 7.5 - 18.0 weight % is the amino acid high content yeast extract which is selected from the aforementioned amino acid high content yeast and the aforementioned amino acid high content yeast which are.本発明は、遊離アミノ酸を高濃度に含有するアミノ酸高含有酵母の製造方法を提供することを目的とする。本発明は、増殖の定常期にある酵母を、液体培地のpHが7.5以上11未満である条件下で液体培養することを特徴とするアミノ酸高含有酵母の製造方法、増殖の定常期にある酵母の液体培地のpHを7.5以上11未満に調整後、さらに培養することを特徴とするアミノ酸高含有酵母の製造方法、前記酵母がサッカロマイセス・セレビシエ(Saccharomyces cerevisiae)、キャンディダ・ユティリス(Candida utilis)であるアミノ酸高含有酵母の製造方法、前記何れかのアミノ酸高含有酵母の製造方法によって得られたアミノ酸高含有酵母、遊離アミノ酸含有量が、乾燥酵母菌体当たり7.5~18.0重量%である前記アミノ酸高含有酵母、前記アミノ酸高含有酵母から抽出されたアミノ酸高含有酵母エキスである。