A feeding regimen for an infant which includes (a) feeding to a newborn infant a first composition including a fat or lipid a protein source, wherein the protein source has from about 72% to about 90% whey and from about 10% to about 28% casein a prebiotic composition and at least about 72 IU/100 kcal of vitamin D, and (b) feeding to a later stage infant a second composition which includes a fat or lipid a protein source, wherein the protein source has from about 50% to about 70% whey and from about 30% to about 50% casein a prebiotic composition and no greater than 70 IU/100 kcal of vitamin D.