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GROWTH HORMONE GENE OF FISH AND POLYPEPTIDE CODED BY SAID GENE
专利权人:
KYOWA HAKKO KOGYO CO LTD
发明人:
SAITO AKIKO,SEKINE SUSUMU,SATO MORIYUKI,ITOU SEIGA
申请号:
JP19850161429
公开号:
JPS6222800(A)
申请日:
1985.07.22
申请国别(地区):
日本
年份:
1987
代理人:
摘要:
NEW MATERIAL:A compound having the peptide sequence expressed by the formula. USE:A growth promoter for fishes. PREPARATION:For example, an mRNA is extracted from an eel pituitary gland body, and a cDNA complementary thereto is synthesized to clone an eel growth hormone gene with a DNA prove corresponding to the amino acid sequence near the N-terminal of the eel growth hormone. The above-mentioned cDNA is then integrated into a vector using a restriction enzyme and DNA polymerase, etc., and the resultant recombinant plasmid is used to transform a microorganism e.g. Escherichia coli. The resultant transformed microorganism is then cultivated in a nutrient culture medium to accumulate a growth hormone polypeptide for fishes in the culture. The formed polypeptide is then collected from the culture to afford the aimed compound expressed by the formula.
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