METHOD FOR QUANTITATIVE DETERMINATION OF PEPTIDES AND DENATURED PROTEINS, NON-COVALENTLY BONDED TO 70 kDa HEAT-SHOCK PROTEIN
- 专利权人:
- GOSUDARSTVENNOE OBRAZOVATEL'NOE UCHREZHDENIE VYSSHEGO PROFESSIONAL'NOGO OBRAZOVANIJA MOSKOVSKAJA MEDITSINSKAJA AKADEMIJA IM. I.M. SECHENOVA FEDERAL'NOGO AGENTSTVA PO ZDRAVOOKHRANENIJU I SOTSIAL'NOMU RAZVITIJU (GOUVPO MMA IM. I.M. SECHENOVA ROSZDRAVA)
- 发明人:
- CHERNIKOV VLADIMIR ALEKSANDROVICH,SAVVATEEVA LJUDMILA VLADIMIROVNA,GOROKHOVETS NEONILA VASIL'EVNA,MAKAROV VLADIMIR ALEKSEEVICH,SEVERIN SERGEJ EVGEN'EVICH
- 申请号：
- RU20080120482
- 公开号：
- RU2384842(C2)
- 申请日:
- 2008.05.26
- 申请国别(地区):
- 俄罗斯
- 年份:
- 2010
- 代理人:
- 摘要：
FIELD: chemistry. ^ SUBSTANCE: invention relates to biochemistry, biopharmacology, laboratory diagnostics and can be used in medicine, immunology and oncology for vaccine standardisation and for research purposes. To realise the method, a solution of adenosine triphosphate is added to the analysed sample until attaining final concentration of 1-5 mM. Incubation is then carried out at temperature 25-40C for 15-60 minutes, with subsequent colorimetric determination of the amount of the formed inorganic phosphate and calculation of the fraction of complexes in the preparation using the formula: The method can be used to standardise vaccines based on HSP70 independent of the method of obtaining the vaccines. The given method is also suitable for use in scientific research, for analysing purity of recombinant protein HSP70, its properties, as well as investigating its reaction with proteins and peptides. ^ EFFECT: use of the invention simplifies quantitative determination and widens the range of determined pept
- 来源网站：
- 中国工程科技知识中心
