Methods of producing a peptide containing an N-substituted amino acid or N-substituted amino acid analog of the present invention include the steps of:preparing an Fmoc-protected amino acid, an Fmoc-protected amino acid analog, or an Fmoc-protected peptide；deprotecting a protecting group which have an Fmoc skeleton of the Fmoc-protected amino acid and such by using a base； andforming an amide bond by adding a new Fmoc-protected amino acid and such；and when the peptide is produced by a solid-phase method, the obtained peptide is cleaved off from the solid phase under conditions of weaker acidity than TFA. Furthermore, at least one side chain of the obtained peptide has a protecting group that is not deprotected under basic conditions and is deprotected under conditions of weaker acidity than TFA.
