A use of a poultry crude protein extract for preparing an anti-fatigue composition is provided. The poultry crude protein extract is obtained by extraction of poultry meat at high temperature and high pressure and the removal of fat by oil and water separation, and the poultry crude protein extract includes at least branched-chain amino acid (BCAA), histidine, threonine, lysine, and phenylalanine, wherein anti-fatigue entails increasing muscle glycogen concentration, helping to increase exercise tolerance, helping to increase blood urea nitrogen metabolism, and helping to inhibit lactic acid production.本發明提供一種禽類粗蛋白萃取物用於製備抗疲勞之組合物的用途，其中該禽類粗蛋白萃取物係一禽類肉品經由高溫高壓萃取，在經由油水分離去除油脂後而獲得，且該禽類粗蛋白萃取物至少包含支鏈胺基酸(Branched- chain amino acid，BCAA)、組胺酸(Histidine)、酥胺酸(Threonine)、離胺酸(Lysine)以及苯丙胺酸(Phenylalanine)；其中該抗疲勞包含提高肌肉肝醣濃度、有助於增加運動耐力、有助於提升血液中尿素氮代謝以及有助於抑制乳酸生成。
