A use of a poultry crude protein extract for preparing an anti-fatigue composition is provided. The poultry crude protein extract is obtained by extraction of poultry meat at high temperature and high pressure and the removal of fat by oil and water separation, and the poultry crude protein extract includes at least branched-chain amino acid (BCAA), histidine, threonine, lysine, and phenylalanine, wherein anti-fatigue entails increasing muscle glycogen concentration, helping to increase exercise tolerance, helping to increase blood urea nitrogen metabolism, and helping to inhibit lactic acid production.
