The present specification discloses a composition for preventing hair loss or promoting hair growth comprising extracellular vesicles derived from lactic acid bacteria as an active ingredient, and a method for producing the extracellular vesicles derived from the lactic acid bacteria. The composition has the effect of preventing and preventing the phenomenon of hair falling out of the scalp, or the phenomenon of hair becoming sparse or thin. In addition, the composition promotes not only the hair growth function for generating new hair or the function for promoting the hair growth, but also the delay from the growth phase to the regression phase of the hair cycle, and the existing hair grows healthy. There is an effect of embodying the function.本明細書には、乳酸菌由来の細胞外小胞を有効成分として含む脱毛防止又は育毛促進用組成物、及び前記乳酸菌由来の細胞外小胞の製造方法が開示される。前記組成物は、頭皮から毛髪が抜ける現象、又は毛髪が疎になる又は細くなる現象を予防し防止する効果がある。また、前記組成物は、新しい毛髪を生成する発毛機能、又は前記発毛を促進させる機能だけでなく、毛髪周期の成長期から退行期への遅延を促進し且つ既存の毛髪が健康に育つようにする機能を具現する効果がある。