A universal serial bus controller and host are adapted for being electrically connected to a universal serial bus device. The universal serial bus host includes: a wiring substrate including a plurality of first substrate contacts and a plurality of second substrate contacts; a universal serial bus connecting port disposed on the wiring substrate via the first substrate contacts; and the universal serial bus controller including a plurality of pins electrically connected to the wiring substrate via the second substrate contacts. The universal serial bus controller and the host can decrease interferences among signals and avoid the complicated layout of the wiring substrate.