Patch (1) for regenerating biological tissues in patients and method of manufacture associated with the same, comprising a collagen membrane (2) which comprises cells with a regenerative character； and at least one reinforcement element (3a, 3b) configured to increase the rigidity of said membrane (2), preventing the deformation of said membrane (2) during the handling thereof； wherein said reinforcement element (3a, 3b) can be a perimeter frame (3a) and/or a crossed attachment (3b) between the ends of said membrane (2)； which provides a patch (1) which can be applied to any type of biological organ with maximum assurances of success as well as reducing the application time.
