Yuelei Shen,Yang Bai,Rui Huang,Xiaofei Zhou,Yuting Hu,Yanan Guo,Jichao Du
申请号：
US16428946
公开号：
US20190343094A1
申请日:
2019.05.31
申请国别(地区):
US
年份:
2019
代理人:
摘要：
Provided is a method for preparing a PD-1 gene-modified humanized animal model. The method utilizes the CRIPSR/Cas9 technique to replace partial fragments of a mouse PD-1 gene with fragments of a human PD-1 gene using homologous recombination by constructing a targeting vector, thereby preparing a gene-modified humanized mouse. This mouse can normally express a PD-1 protein containing the functional domain of the human PD-1 protein, and can be used as an animal model for mechanism research regarding PD-1, PD-L1 and other signals, for screening regulators, and for toxicological research. The method has an important and high application value in studies on functions of the PD-1 gene and in the development of new drugs.
