PROBLEM TO BE SOLVED: To shorten the production time of good gut bacteria producing substances which takes a long time.SOLUTION: A culture medium preparation step of preparing a culture medium by heat sterilizing a culture medium feedstock comprising soybean flour and water which has been put into a culture tank. By using soybean flour in the culture medium various nutrients which are abundantly included in soy beans can be given to lactic acid bacteria and/or bifidobacteria and the growth and growth rate of the lactic acid bacteria and/or bifidobacteria can be increased. Thereafter, a culturing step of culturing the lactic acid bacteria and/or bifidobacteria by stirring and warming the culture product in which lactic acid bacteria and/or bifidobacteria has been added to the culture medium in the culture tank is performed. By stirring the culture product in the culture medium, nutrients can be evenly distributed to the lactic acid bacteria and/or bifidobacteria and even a large amount of culture product can be quickly cultured.SELECTED DRAWING: Figure 3COPYRIGHT: (C)2018,JPO&INPIT【課題】長時間かかる善玉菌生産物質の製造時間を短縮する。【解決手段】培養槽内に大豆粉と水を含む培地原料を投入し加熱滅菌して培地を調製する培地調製工程を行う。培地に大豆粉を用いることで、大豆に豊富に含まれる各種の栄養素を乳酸菌及び/又はビフィズス菌に与えることができ、乳酸菌及び/又はビフィズス菌の生育、増殖速度を増すことができる。次に、前記培養槽内の培地に乳酸菌及び/又はビフィズス菌を添加した培養物を攪拌しつつ加温し前記乳酸菌及び/又はビフィズス菌を培養する培養工程とを行う。培養中に培養物を攪拌することで、乳酸菌及び/又はビフィズス菌に栄養素を満遍なく行き渡らせることができ、大量の培養物であっても迅速に培養することができる。【選択図】図3