FA Zhermejn (US),ФА Жермейн (US),LI Chingvehj V. (US),ЛИ Чингвэй В. (US),FA ZHERMEJN,ФА Жермейн,LI CHINGVEHJ V.,ЛИ Чингвэй В.
申请号：
RU2010126539/10
公开号：
RU0002514148C2
申请日:
2008.12.01
申请国别(地区):
RU
年份:
2014
代理人:
摘要：
FIELD: medicine, pharmaceutics.SUBSTANCE: invention relates to immunology. Described are antibodies against VEGF, one on which contains complementary regions with amino acid sequences SEQ ID NO:1, 2, 3, 4, 6 and 7, another contains complementary regions with amino acid sequences SEQ ID NO:1, 2, 3, 5, 6 and 7, disclosed in description. Also described are polynucleotides, coding said antibodies； espression vectors, containing said polynucleotides, and host cells, intended for obtaining antibodies in accordance with the claimed invention. Claimed is method of obtaining antibodies against VEGF, which includes expression of vector in host cell and separation of antibody. Disclosed is method of obtaining immunocongugate of antibody against VEGF, which includes conjugation of antibody with drug or cytotoxic agent. Described is method of VEGF identification, which includes identification of complex VEGF-antibody against VEGF in biological sample. In addition, described are compositions for treatment of VEGF-associated disease, one of which contains efficient quantity of antibody against VEGF, and another - efficient quantity of polynucleotide, coding said antibody. Also disclosed are methods of: 1) treating tumour, cancer or VEGF-associated cell proliferative disease； 2) inhibition if angiogenesis in subject and 3) inhibition of vascular permeability； consisting in introduction to subject of efficient quantity of antibody against VEGF in accordance with claimed invention.EFFECT: invention makes it possible to obtain antibodies against VEGF and apply them for treatment of VEGF-associated diseases.41 cl, 16 dwg, 2 tbl, 5 exИзобретение относится к области иммунологии. Описаны антитела против VEGF, одно из которых содержит комплементарные регионы с аминокислотными последовательностями SEQ ID NO:1, 2, 3, 4, 6 и 7, другое содержит комплементарные регионы с аминокислотными последовательностями SEQ ID NO:1, 2, 3, 5, 6 и 7, раскрытыми в описании. Также описаны полинуклеотиды, коди
