PROBLEM TO BE SOLVED: To provide urine progenitor cells, and to provide a method for producing a culture of urine progenitor cells from a urine sample, for urological tissue engineering and cell therapy.SOLUTION: The cells may be selected based upon the use of a selective cell medium, based upon morphology, and/or by selecting cell-specific markers. The invention provides an isolated urine progenitor cell that is c-kit positive and can differentiate into urothelium, smooth muscle, endothelium or interstitial cells. The invention also provides methods of use of the urine progenitor cells, wherein the cells are seeded onto a tissue scaffold. The invention also provides methods of treating a subject in need thereof comprising providing a bladder tissue substrate that includes differentiated UPCs and transplanting the substrate into the patient. Finally, the invention provides kits that include a container suitable for the transport of a urine sample media one or more types of antibiotics a package for holding the container, media, and antibiotics and optionally, instructions for use.SELECTED DRAWING: Figure 1COPYRIGHT: (C)2016,JPO&INPIT【課題】泌尿器組織工学及び細胞療法のため、尿中前駆細胞、および尿サンプルからの尿中前駆細胞の培養を生産する方法の提供。【解決手段】細胞は、選択細胞培地の使用に基づいた選別、形態学に基づいた選別、及び/又は細胞特異的マーカーによる選別を行い、c-kit陽性であり、尿路上皮細胞、平滑筋細胞、内皮細胞、または間質細胞に分化可能である、分離された尿中前駆細胞。尿中前駆細胞の使用方法であって、細胞が組織足場上に播種され、治療を必要とする被験体の治療方法であって、分化したUPCを含む膀胱組織基質を提供するステップと、およびその基質を患者の体内に移植するステップとを含む方法。最後に、尿サンプルの輸送に適した容器と、培地と、1種類以上の抗生物質と、前記容器、培地、および抗生物質を保持するための包装材料と、場合により使用説明書とを含むキット。【選択図】図1
