Embodiments of the invention relate to human, non-mesenchymal c-kit positive lung stem cells negative for the CD44, CD73 and CD105 markers of the mesenchymal stromal cell lineage (non-mhLSCs) and their therapeutic use in the treatment and/or prevention of lung diseases or disorders. Provided herein are compositions comprising non-mhLSCs and methods of preparing and using non-mhLSCs for the treatment and/or prevention of lung diseases or disorders.