Williams David Hugh;Turner Arthur Keith;Wain John Richard
发明人:
Williams David Hugh,Turner Arthur Keith,Wain John Richard
申请号：
US201715818579
公开号：
US2018135041(A1)
申请日:
2017.11.20
申请国别(地区):
美国
年份:
2018
代理人:
摘要：
Described is a process for producing a mutant bacterium which exhibits improved survival and/or growth under a selected growth condition, the process comprising the steps of: (a) generating a pool of mutant bacteria by transposon mutagenesis with an activating transposon (TnA), wherein the TnA comprises a promoter capable of increasing transcription of a gene at or near its insertion site; (b) growing bacteria from the mutant pool under the selected growth condition and under one or more reference conditions to produce two or more test cultures; and (c) comparing the distribution of TnA insertions between test cultures to identify a first class of genes which are disadvantageous for growth and/or survival under the selected growth condition and a second class of genes which are advantageous for growth and/or survival under the selected growth condition.
