UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION;CHAPPELL, JOE;YEO, YUNSOO;TAKAHASHJI, SHUNJI
发明人:
CHAPPELL, JOE,YEO, YUNSOO,TAKAHASHJI, SHUNJI
申请号：
WO2006US02265
公开号：
WO2006079020(A3)
申请日:
2006.01.19
申请国别(地区):
世界知识产权组织国际局
年份:
2008
代理人:
摘要：
Isolated and purified nucleic acid molecules encoding Hyoscyamus muticus premnaspirodiene oxidase (HPO) have been prepared by gene cloning. These nucleic acid molecules encode the oxidase enzyme which can catalyze the hydroxylation of valencene to ??-nootkatol and nootkatone, which is industrially important and used in food products. Also within the scope of the invention are vectors including the nucleic acid molecules, host cells transformed or transfected by the vectors, and methods of producing proteins by expression of the nucleic acid molecules in the host cells. Further within the scope of the invention are methods of producing an oxidized terpene substrate from an unoxidized terpene substrate using proteins or expressed nucleic acid molecules according to the invention.
