An immunogenic agent suitable for preventing, treating or immunizing against one or a plurality of different pathogen comprises an immunogenic agent which comprises one or a plurality of pathogen-derived proteins, fragments, variants or derivatives thereof displayed on a lipid vesicle and a carrier protein such as diptheria toxoid located in an intravesicular space. The immunogenic agent may be suitable for intranasal administration and may be capable of eliciting a mucosal immune response. The immunogenic agent may further comprise an activator of innate immunity such as trehalose-6,6′-dibehenate and/or a bile salt such as sodium deoxycholate. The one or plurality of pathogens may be group A streptococcus, viruses or hookworms.本發明提供一種適用於針對一或複數個不同病原的預防、治療或免疫之免疫原性試劑,其包含含有呈現於脂囊泡上之一或複數個病原衍生蛋白質、其片段、變異體或衍生物、及位於囊內空間的如白喉類毒素的載體蛋白之免疫原性試劑。免疫原性試劑可適用於鼻內施予且其可誘發黏膜免疫反應。免疫原性試劑可進一步包含例如海藻糖-6,6′-二山萮酸酯及/或膽鹽,例如脫氧膽酸鈉之先天性免疫活化劑。一或複數個病原可為A群鏈球菌、病毒或鉤蟲。無
