Commonwealth Scientific and Industrial Research Organisation
发明人:
Li, Zhongyi,Morrel, Mathew Kennedy,Jobling, Stephen Alan
申请号：
AU2012212404
公开号：
AU2012212404A1
申请日:
2012.02.03
申请国别(地区):
AU
年份:
2013
代理人:
摘要：
Barley grain, plants and parts thereof producing same and methods of producing and using same are described, the grain comprising (a) starch (b) a first genetic variation which is (i) an induced mutation in an endogenous gene encoding a starch synthase HI (SSIII), or (ii) a transgene which encodes an RNA molecule which reduces expression of an endogenous gene encoding an SSIII, and (c) a reduced level or activity of SSIII protein relative to the level or activity in barley grain lacking the first genetic variation. The grain may further comprise a second genetic variation in a gene encoding a protein involved in starch synthesis, starch catabolism, starch phosphorylation or non-starch carbohydrate synthesis. The second genetic variation is illustratively (i) a mutation in a gene encoding a starch synthase II (SSII), in some embodiments a starch synthase Ila (SSIIa), or (ii) a transgene which encodes an RNA molecule which inhibits expression of a gene encoding an SSII, preferably an SSIIa. The grain illustratively comprises (i) an allele of SSIIIa other than an allele comprising the nucleic acid sequence of SEQ ID NO: 3 and (ii) an allele of SSIIa other than an allele comprising the nucleic acid sequence of SEQ ID NO: 33. The SSIII is illustratively SSIIIa. The SSII is illustratively SSIIa.
