КРАМЕР Брайан К. (US),БРАУН Лаура Дж. (US),КИХМ Энтони Дж. (US)
申请号：
RU2013156357/15
公开号：
RU2013156357A
申请日:
2012.05.17
申请国别(地区):
RU
年份:
2015
代理人:
摘要：
1. A method of treating a disease or condition associated with degenerative changes in the intervertebral disc, comprising administering a pharmaceutical composition comprising a hydrogel and an isolated homogeneous population of cells derived from human umbilical cord tissue into the intervertebral disc in an amount effective to treat such a disease or condition, the tissue umbilical cord, essentially free of blood, and moreover, the selected homogeneous population of cells capable of self-renewal and reproduction in culture, has the potential for differentiation tion and does not express CD117 or telomerazu.2. The method of claim 1, wherein the isolated cell population has one or more of the following characteristics: (a) expresses a reticulon, a chemokine receptor 3 ligand and / or a granulocyte chemotactic protein； (b) does not produce CD31, CD34 and HLA-DR； ( c) expresses, in relation to fibroblasts, mesenchymal stem cells or bone marrow cells of the human iliac crest, elevated levels of interleukin 8 and reticulon 1； and (d) expresses CD10, CD13, CD44, CD73 and CD90.3. The method of claim 1, wherein the pharmaceutical composition is administered by injection. The method of claim 1, wherein the pharmaceutical composition further comprises at least one other type of cell and / or at least one agent. The method of claim 4, wherein the at least one agent is a trophic factor. The method of claim 5, wherein the trophic factor is selected from the group consisting of TGF-beta, GDF-5, PDGF-BB, and TIMP1.7. The method of claim 4, wherein at least one other type of cell is obtained by genetic engineering methods in such a way that they express at least one �1. Способ лечения заболевания или состояния, связанного с дегенеративными изменениями межпозвоночного диска, содержащий введение фармацевтической композиции, содержащей гидрогель и выделенную однородную популяцию клеток, полученных из ткани пуповины человека, в межпозвоночный диск в количестве, эффективном для лечени
