PROBLEM TO BE SOLVED: To provide a chalcone 2'-O-glucosyltransferase (Ch 2'GT) gene, to provide a method for utilizing the same, especially a method for changing the flower color of a plant. SOLUTION: All RNAs were extracted from flower petals in the flower buds of Dianthus caryophyllus [A66] putting forth yellow flowers and Dianthus caryophyllus [8358-01] putting forth orange color flowers, and mRNAs were prepared. Further, cDNAs were synthesized. cDNAs as candidates for the Ch 2'GT were isolated by PCR screening treatment using a degeneracy primer. Subsequently, the activities of encoded proteins were measured from the cDNAs. As the result, it was found that there were two kinds of the cDNAs for producing a chalconnaringenin glucoside from chalconnaringenin as a substrate. The flower color of a plant can be changed by the utilization of the DNAs encoding the Ch 2'GT. COPYRIGHT: (C)2005,JPO&NCIPI
