The present disclosure provides a culture method for improving amount of triterpenoids in inonotus obliquus, and the culture method includes the following steps. First, a strain of the inonotus obliquus is inoculate to a solid medium for producing mycelium of the inonotus obliquus. When an area of the cultivated mycelium of the inonotus obliquus is up to a first predetermined value, a first nutrient solution is added to the solid medium. When the area of the cultivated mycelium of the inonotus obliquus is up to a second predetermined value, a brightness and a temperature of the environment, where the mycelium of the inonotus obliquus is cultured, are lowered until a second nutrient solution is added to the solid medium. When the area of the cultivated mycelium of the inonotus obliquus is up to a third predetermined value, a harvesting step is performed. Therefore, the amount of the triterpenoids in the inonotus obliquus can be improved through the present disclosure.本發明提供一種用以提高白樺茸中三萜類化合物含量之培養方法,其包含接種步驟、添加第一營養液步驟、第一培養環境調整步驟、添加第二營養液步驟、第二培養環境調整步驟以及採收步驟。首先,將白樺茸菌株接種至固態培養基以培養出白樺茸菌絲體。當白樺茸菌絲體達第一預設面積時,添加第一營養液。當白樺茸菌絲體達第二預設面積時,降低培養環境之亮度與溫度後。接著,在添加第二營養液後,回復培養環境之亮度與溫度,並待白樺茸菌絲體達第三預設面積時採收。藉此,本發明可有效提升白樺茸菌絲體產物中三萜類化合物的含量。S100、S102、S104、S106、S108、S110‧‧‧步驟
