ATARES-REAL, Sergio,MARTIN-PEREZ, Julia,ROMERO-LOPEZ, Joaquín,SALAET-MADORRAN, Ignasi,MARQUES-MASCARELL, Ramón,ALIGUE, Rosa
申请号：
EPEP2015/075834
公开号：
WO2016/071458A1
申请日:
2015.11.05
申请国别(地区):
EP
年份:
2016
代理人:
摘要：
The invention generally relates to novel and improved nucleic acid sequences encoding a polypeptide or protein having the enzymatic activity of a phytase and methods for effectively expressing such enzymes in a host cell. In a first aspect, the present invention relates an isolated nucleic acid molecule i) comprising the nucleotide sequence according to SEQ ID NO: 1 encoding a protein with phytase activity or ii) having a sequence identity of at least 83 % to a nucleotide sequence according to SEQ ID NO: 1 encoding a protein with phytase activity. The present invention also relates to an expression construct or a vector comprising the above mentioned isolated nucleic acid molecule. The present invention further relates to a method of producing a protein having the enzymatic activity of a phytase comprising the steps of: a) introducing into a host cell a vector comprising: i) elements regulating the expression that are functional in the host cell； and ii) operatively linked thereto a nucleic acid molecule according to the invention； b) cultivating the host cells obtained in step a) under conditions suitable for expression of the protein, and optionally c) recovering the protein produced in step b) from the cell culture. The present invention further relates to proteins obtained by the method according to the invention and the use thereof for the manufacture of additives for animal feed.L'invention porte d'une manière générale sur des séquences d'acides nucléiques nouvelles et améliorées codant un polypeptide ou une protéine ayant l'activité enzymatique d'une phytase, ainsi que sur des procédés pour exprimer de manière efficace ces enzymes dans une cellule hôte. Dans un premier aspect, la présente invention concerne une molécule d'acide nucléique isolée i) comprenant la séquence nucléotidique selon la SEQ ID NO : 1 codant une protéine ayant une activité de phytase ou ii) ayant une identité de séquence d'au moins 83 % avec une séquence nucléotidique selon la SEQ ID NO
