The present invention provides a method for purifying Antrodin C, including extracting the mycelium of Antrodia with an organic solvent to obtain an extract, and loading the extract into a high performance centrifugal partition chromatography (HPCPC) to obtain Antrodin C.本發明為一種純化Antrodin C之方法,包括以有機溶劑萃取薄孔菌屬(Antrodia)的菌絲體,獲得一萃取物,以及將該萃取物注入高效離心分配層析儀(high performance centrifugal partition chromatography,HPCPC),以獲得Antrodin C。201‧‧‧有機溶劑萃取薄孔菌屬菌絲體202‧‧‧HPCPC分離程序