A method of making a multi-layer pad for use in determining an analyte concentration, the method comprising the acts of:immersing a first layer of the multi-layer pad in a first solution including at least one enzyme, the first layer having a first surface and a second surfaceremoving the first layer from the first solutiondrying the first layer such that the at least one enzyme is physically absorbed in the first layerafter drying the first layer, applying a second solution to at least one of the first surface and the second surface of the first layer andchemically cross-linking the second solution to the first layer, the chemically cross-linked second solution forming at least one outer polymeric layer.
