The United States of America;as represented by the Secretary;Department of Health and Human Services
发明人:
Yong-Chen Lu,Peter Fitzgerald,Zhili Zheng,Steven A. Rosenberg
申请号：
US16495508
公开号：
US20200056237A1
申请日:
2018.03.28
申请国别(地区):
US
年份:
2020
代理人:
摘要：
Disclosed are methods of isolating paired T cell receptor (TCR) alpha and beta chain sequences, or an antigen-binding portion thereof. Also disclosed are methods of automatically identifying the TCR alpha and beta chain V segment sequences and CDR3 sequences of a TCR having antigenic specificity for a mutated amino acid sequence encoded by a cancer-specific mutation. Methods of preparing a population of cells that express paired TCR alpha and beta chain sequences, or an antigen-binding portion thereof, are also disclosed. Isolated pairs of TCR alpha and beta chain sequences and isolated populations of cells prepared by the methods are also disclosed.
