OSBORNE, ROSEMARIE,PERKINS, MARY, ANN,ROBERTS, DEIRDRE, ANNE
申请号：
WO1993US00737
公开号：
WO9317336(A1)
申请日:
1993.01.27
申请国别(地区):
世界知识产权组织国际局
年份:
1993
代理人:
摘要：
A technique for in vitro testing of ocular and dermal irritants is disclosed. The process involves the topical application of liquid, solid granular or gel-like materials (e.g. cosmetics) to a cell culture and then evaluating the cytotoxicity of the material. Cell cultures of human skin without a stratum corneum and having a histologic similarity to the eye are used. Irritation is evaluated by measuring cell viability using MTT assay (based on the reduction of a tetrazolium dye by functional mitochondria), or release of LDH or PGE2. A unique method of applying test materials which are not water soluble is described.
