PROCEDIMIENTO DE PREPARACIÓN DE UNA OLEORRESINA PROVENIENTE DE UN ALGA ROJA, QUE MANTIENE LA CAPACIDAD DE INDUCIR LA ACTIVIDAD TRANSCRIPCIONAL DEL RECEPTOR NUCLEAR PPARy,
BRONFMAN, MIGUEL,BRONFMAN, Miguel,BRONFMAN FRANCISCA,BRONFMAN Francisca,PINTO, CLAUDIO,PINTO, Claudio,PISSANI, CLAUDIA,PISSANI, Claudia,PAREDES MARTÍNEZ, MARÍA JOSÉ,PAREDES MARTÍNEZ, María José
申请号：
CLCL2013/000031
公开号：
WO2014/186913A1
申请日:
2013.05.23
申请国别(地区):
WO
年份:
2014
代理人:
摘要：
The present invention relates to a method for preparing an oily extract originating from a red alga, preferably Gracilariachilenis. This process successfully maintains, in the oily extract, a high capacity to induce PPAR-gamma transcriptional activity. This process comprises the steps of: I) Extraction of the fresh alga and transport at low temperature: wherein the live, whole alga is gathered by being torn from its attachment to the seabed substrate in order then to be transferred cold (approximately 4°C) II) Preparation and chopping-up of the alga: comprising the washing of the alga with PBS (pH 7.4), extraction by centrifugation and storage under vacuum at ‑20°C. Thawing to 4°C and further washing with PBS (pH 7.4), drying by centrifugation and chopping-up of the alga with a stainless-steel pound knife into fragments of between 1-3 mm, for subsequent freezing briefly at ‑20°C for lyophilization thereof III) Freeze-drying: lyophilization for 24 hours at ‑50°C and 0.014 mbar and storage under vacuum at ‑20°C IV) Spraying with batchwise solid-liquid extraction of the oleoresin: the lyophilized alga is brought to ambient temperature (20-25°C) and then ground to a fine powder with an average size of less than ½ mm. A quantity of powder is placed in a flask and mixed with an appropriate quantity of solvent (dichloromethane) in order for a batchwise solid-liquid extraction to be performed. This mixture is saturated in a gaseous nitrogen atmosphere and sealed, then undergoing horizontal agitation at 34°C for 30 min. The mixture is then decanted and filtered on paper and the liquid phase is received in a flask. The solid phase is again resuspended in a liposoluble solvent, preferably dichloromethane, the earlier extraction process being repeated. The total liquid phase or extract is concentrated in a rotary evaporator, which removes the solvent, leaving the oleoresin. This product is dried under nitrogen gas to constant weight and is then resuspended in a minimum volume o
