PROBLEM TO BE SOLVED: To provide an efficient process for extractingβ-glucan. SOLUTION: The process of manufacturingβ-glucan comprises extractingβ-glucan from fruit bodies of Lentinus edodes prepared by a culturing method, where after cutting buds during the growth period of Lentinus edodis, the environment temperature is controlled to keep at 5-18°C. COPYRIGHT: (C)2006,JPO&NCIPI