The "method for preparing yeast DNA raw material for promoting keratin metabolism and blemish spotting" of the present invention includes the following steps in order: yeast cell preparation, cell disruption and yeast DNA extraction, DNA enzyme shearing, purification of small fragments of yeast DNA, Preparation of Saccharomyces cerevisiae DNA, screening of active single-stranded yeast ribonucleic acid using a gene chip for promoting keratin metabolism and blemishes, and obtaining raw materials for promoting keratin metabolism and bleae yeast DNA. The keratin metabolism-promoting and spotted yeast DNA material obtained in this case can be combined with the mRNA of the ROCK gene to make the gene ineffective, thereby promoting melanin metabolism by keratinocytes, and promoting skin keratin metabolism and blemishes.本發明「促進角質代謝及淡斑之酵母DNA原料製法」，包括依序進行下述步驟：酵母菌菌體製備、菌體破碎及酵母DNA萃取、DNA酵素剪切、純化小片段酵母DNA、單股酵母DNA製備、利用促進角質代謝及淡斑基因晶片篩選活性單股酵母核糖核酸、取得促進角質代謝及淡斑酵母DNA原料。藉由本案所取得之促進角質代謝及淡斑酵母DNA原料，可與ROCK基因之mRNA結合，使該基因失去作用，進而促進角質細胞代謝黑色素，達到促進皮膚角質代謝及淡斑等效果。
