The invention is suitable for the field of communication services and provides a real-time detection method of a physiological signal. The method includes the steps that a timestamp sent by a communication terminal is received by a detection terminal, and synchronous adjusting is performed on a clock according to the timestamp the detection terminal periodically detects the physiological signal of a user the detection terminal sends the physiological signal to the communication terminal in a wireless mode. The physiological signal includes physiological data and the timestamp. According to the technical scheme, the real-time detection method and system of the physiological signal have the advantage of real-time monitoring.發明適用通信服務領域,提供了一種生理信號的即時檢測方法,該方法包括:檢測終端接收通信終端發送的時間戳記,依據該時間戳記對時鐘進行同步調整;檢測終端週期性的檢測使用者的生理信號;檢測終端將該生理信號通過無線方式發送給通信終端,該生理信號包括:生理數據和時間戳記。本發明提供的技術方案具有即時監測監控的優點。