The invention relates to a 3’-isoflavone glycoside having whitening and anti-aging effects, a preparing method and a use thereof. The preparing method comprises the steps of transforming a cyclic recombinant plasmid into a suitable microbial expression system to form a gene recombinant microorganism, wherein the cyclic recombinant plasmid containing a tyrosinase gene fromBacillus megaterium and reacting the gene recombinant microorganism with a reaction solution containing an isoflavone glycoside for a period of time to biotransform the 3’-isoflavone glycoside. Accordingly, the 3’-isoflavone glycoside can be used to inhibit production of melanin and free radicals by applying an effective does to a skin, so as to achieve efficacy of whitening and anti-aging.本發明有關於一種具美白與抗老化之3’-羥基異黃酮糖苷及其製備方法與用途,所述製備方法包括將一環狀重組質體置於一適合的微生物表達系統中,以形成一基因重組微生物,其中環狀重組質體係包含來自巨大芽孢桿菌(Bacillus megaterium)之酪胺酸酶(tyrosinase)基因;利用上述基因重組微生物於含有異黃酮糖苷(isoflavone glycoside)之反應液作用一段時間,以產生3-羥基異黃酮糖苷;藉此,施予一有效劑量之3’-羥基異黃酮糖苷於皮膚,可抑制黑色素生成與抑制自由基產生,達到美白與抗老化之用途。
