CHANG, TE SHENG,张德生,張德生,TSAI, YA TING,蔡雅婷,蔡雅婷,DING, HSIOU YU,丁秀玉,丁秀玉,CHIANG, CHIEN MIN,江建民,江建民
申请号：
TW105100958
公开号：
TW201725267A
申请日:
2016.01.13
申请国别(地区):
TW
年份:
2017
代理人:
摘要：
The invention relates to a method of manufacturing methoxy-isoflavones by biotransformation and use thereof. The method comprises synthesizing a nucleic acid sequence encoding a SpOMT2884 protein ofStreptomyces peucetius cloning the nucleic acid sequence into an expression vector to form a cyclic recombinant plasmid transforming the cyclic recombinant plasmid into a microbial expression system (Escherichia coli) and incubating the microbial expression system in a medium containing 8-hydroxydaidzein therein for generating methoxy-isoflavones. Accordingly, the acquired methoxy-isoflavones can be used to inhibit melanogenesis.本發明係有關於一種甲氧異黃酮(Methoxy-isoflavones)的生物轉換製備方法與用途,製備方法包括:合成具有編碼(encoding)鏈黴菌(Streptomyces peucetius) SpOMT2884蛋白之核酸序列;將核酸序列接合至表現質體以形成環狀重組質體;將環狀重組質體置於微生物表達系統(大腸桿菌,Escherichia coli)中,培養於含有8-羥基大豆苷元(8-hydroxydaidzein)的培養基,使環狀重組質體表現以產生甲氧異黃酮;藉此,所得之甲氧異黃酮具有抑制黑色素形成之用途。(S1)‧‧‧步驟一(S2)‧‧‧步驟二(S3)‧‧‧步驟三(S4)‧‧‧步驟四
