Compositions and methods for cleaning, disinfection, sterilization, and decontamination of surfaces and objects are provided. In particular, a hyperprotonation composition is described that comprises a surfactant, one or more emulsifiers, a biocide, and a weak acid and is effective to disrupt both the microbial biofilm defenses as well as the microbes within. Methods of applying the hyperprotonation compositions to contaminated surfaces, equipment, fabrics, food, and human or animal tissue to disrupt the microbial biofilms and eradicate the microbes within are also disclosed.
