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PROCESS AND KIT FOR PREDICTING ANTIBIOTIC RESISTANCE AND SUSCEPTIBILITY OF BACTERIA
专利权人:
Idgenomics, Inc.
发明人:
CHATTOPADHYAY, Sujay,SOKURENKO, Evgeni
申请号:
EP20160756508
公开号:
EP3262198(A1)
申请日:
2016.02.26
申请国别(地区):
欧洲专利局
年份:
2018
代理人:
摘要:
There is disclosed a PCR-based test kit and PCR process for identification of multiple clonal sub-species lineages of infectious bacteria, such as uropathogenic E. coli causing cystitis, pyelonephritis and urosepsis, for the purposes of predicting antibiotic resistance of the bacteria. More specifically, there is further disclosed a SNP (single nucleotide polymorphism) identification process that simultaneously detect compilations of the presence of absence of predictive SNPs within mutated loci of infectious bacterial clonal subspecies variants, such as the fumC/fimH loci of the E. coli bacterium. This disclosure provides a PCR detection kit incorporating a SNP compilation that forms a BFC (Binary Footprint Code) that allows for rapid identification of multiple infectious bacterial clonotypes based on their SNP footprint. More specifically there is disclosed a clonotyping method for clonal typing E. coli and predicting antibiotic susceptibility, comprising (a) providing forward primers and reverse primers fo
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