The present invention relates to a manufacturing method of an anti-fog film, comprising: alkalization the main component of the anti-fog film, tri-acetyl cellulose neutralization and water-washing drying the feature of the present invention is characterized in that the concentration of the alkali etching solution for alkalization of the tri-acetyl cellulose is within 6%~9%. The present invention also relates to an anti-fog film manufactured thereof and the use of the anti-fog film as a gauze mask, an eyeshade or a lens coating film.本發明係提供一種防霧膜片以及其製造方法,步驟包含:對該防霧膜片之一主體成分三醋酸纖維進行鹼化處理;進行中和水洗步驟;烘乾;其特徵在於,該三醋酸纖維係經由濃度6至9%之鹼性蝕刻溶液進行鹼化處理,以及一種以該製造方法製成之防霧膜片與該防霧膜片用於口罩、眼罩或鏡片貼膜之用途。