INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY; CHINESE ACADEMY OF SCIENCES
发明人:
申请号：
ARP180103816
公开号：
AR113433A1
申请日:
2018.12.21
申请国别(地区):
AR
年份:
2020
代理人:
摘要：
The current problems are related to the field of genetic engineering. In particular, the current practice is related to plant-based editing methods. More specifically, this paper relates to a method for editing extremely effective substrates for objective sequences of plant genomes (e.g. culture media) from a to G by RNA guided deoxypyrethrin adenosine mixed proteins and plants and their offspring produced by this method. Requirement 1: a system for editing the basis of an objective sequence in a plant genome,It includes at least the following points: (1) fusion protein and a guide RNA for base editing； (2) an expression structure, including a nucleoside sequence, encoding a fusion protein and a guide RNA for base editing； (3) fusion protein and a guide RNA for base editing. 1. Establish an expression structure including the nucleoside sequence of the compiled guide RNA； 4) an expression structure including the nucleoside sequence, which is the basis for editing and coding a fusion protein, and an expression structure including the nucleoside sequence of the compiled guide RNA；（5） An expression structure includes a nucleoside sequence, which encodes a fusion protein for base editing, and a nucleoside sequence encodes a guide RNA； When fusion proteins for base editing include melter proteins of a discontinued ribozyme and DNA dependent deoxyadenosine, the guiding RNA can guide fusion proteins to an objective sequence of the plant genome for base editing, Thus, molten proteins for basic editing result in the substitution of G for one or more a's in the target sequence.La presente se relaciona con el campo de la ingeniería genética. En particular, la presente se relaciona con un método para editar bases en plantas. Más específicamente, la presente se relaciona con un método para editar bases de A a G altamente eficaz para una secuencia objetivo en el genoma de una planta (por ejemplo, una planta de cultivo) a través de una proteína de fusión CRISPR-adenina desaminasa d
