Methods of producing a Protein of interest in a heterologous Cell, to increase the productivity of a Specific Cell Membrane or secreted Protein of interest in a cell, to increase the transfection efficiency of a cell that expresses a Membrane or Protein of interest Cretada in a cell and to identify a Modulator of the function of Protein Domain start with,Expression Vectors, Cells, Proteins, Pharmaceutical compositions and their uses
A method of producing a Protein of interest in a heterologous Cell comprising) increase the expression or activity of a Protein has an Amino Acid Sequence comprising a Lipid Transfer Domain related regulatory protein of steroid Acute og u00e9nesis (START) or a derivative or Mutant thereof,Where the Protein has an Amino Acid Sequence does not include Ceramide transfer protein (CERT)Where the derivative is not a polypeptide Molecule at least 70% identical in sequence with the original Sequence or complementary Sequence or CERT CERT Said derivative is not a sequence homologous to a different Agency and where the Mutant is not based on amendments of CERT sequences by substitutionInsertion or removal of one to ten Amino Acids； and (b) make the expression of the Protein of interest.Methods to increase productivity of a Specific Cell Membrane or secreted Protein of interest in a cell, to increase the efficacy of transfection of a cell that expresses a Membrane or secreted Protein of interest in a cell and to identify a Modulator The role of Protein Domain start with, Expression Vectors, Cells, Proteins,Pharmaceutical compositions and their uses.Un método de producción de una proteína de interés heteróloga en una célula que comprende a) aumentar la expresión o actividad de una proteína que tiene una secuencia de amino ácidos que comprende un dominio de transferencia de lípido relacionado con la proteína reguladora de la esteroidogénesis aguda (START) o un derivado o mutante del mismo, en donde la proteína que tiene una secuencia de aminoácidos no comprende proteína de transferencia de ceramida (CERT), en donde el derivado no es una molécula de polipéptido al menos 70% idéntica en secuencia con la secuencia original CERT o su secuencia complementaria o dicho derivado no es una secuencia CERT homóloga de un organismo diferente y en donde dicho mutante no se basa en modificaciones dirigidas de secuencias CERT por sustitución, inserción o eliminación de uno a diez aminoácidos；
