Fluorescence observation apparatus of the present invention, for emitting fluorescent substance of the plurality of light sources capable of emitting section, a plurality of pump lights and the plurality of pump lights for exciting each of the plurality of fluorescent materials, and reference light and generating respective image signals corresponding to the imaging section for imaging the plurality of fluorescent emitted by the, the, the reflected light of the reference light, a plurality of fluorescent taken by the imaging unit and to the reflected light of the reference light a brightness detection unit for detecting respectively, and brightness of the image signal corresponding to the image generation unit that, the brightness of the plurality of image signals corresponding to a plurality of fluorescent and reflected light of the reference light, and detecting the brightness detection unit Based on the results, based on the brightness of the image signal corresponding to the reflected light of the reference light, a portion brightness adjustment for adjusting the brightness of the image signal of at least one of the plurality of image signals corresponding to a plurality of fluorescent , I have a.本発明の蛍光観察装置は、複数の蛍光物質をそれぞれ励起させるための複数の励起光と、参照光と、を出射可能な光源部と、複数の励起光を前記複数の蛍光物質に対して出射することにより発せられる複数の蛍光と、参照光の反射光と、を撮像する撮像部と、撮像部により撮像された複数の蛍光と、参照光の反射光と、に応じた画像信号をそれぞれ生成する画像生成部と、複数の蛍光に応じた複数の画像信号の明るさと、参照光の反射光に応じた画像信号の明るさと、をそれぞれ検出する明るさ検出部と、明るさ検出部における検出結果に基づき、参照光の反射光に応じた画像信号の明るさを基準として、複数の蛍光に応じた複数の画像信号のうちの少なくとも一の画像信号の明るさを調整する明るさ調整部と、を有する。
