Observation is performed by a fluorescence image highly quantitative by removing sufficient distance dependencies. Lighting unit with (10) light source illuminating light (X) the examination site and irradiated with excitation light (20), to obtain a fluorescent image capturing fluorescence generated examination site in (X) by irradiation of the excitation light White light imaging unit fluorescence imaging unit that (42), obtains a reference image captured return light returning from the (X) the examination site by the irradiation of the illumination light (44), the distance of the viewing distance of the brightness of the fluorescence image die to obtain a corrected fluorescent image by the power of the luminance value of the fluorescence image in the reciprocal of first index obtained by power approximation characteristics, can be obtained by power approximation distance characteristics in viewing distance of the luminance of the reference image image correction unit which acquires a corrected reference image by the power of the luminance value of the reference image by the inverse of an exponential of 2, to obtain the (K) corrected fluorescence image is divided by the correction reference image corrected for fluorescence image (60 I provide the (101) fluorescence observation apparatus and a).距離の依存性を十分に除去して定量性の高い蛍光画像によって観察を行う。観察対象部位(X)に照明光および励起光を照射する光源(10)を備える照明ユニット(20)と、励起光の照射により観察対象部位(X)において発生した蛍光を撮影し蛍光画像を取得する蛍光撮像部(42)と、照明光の照射により観察対象部位(X)から戻る戻り光を撮影し参照画像を取得する白色光撮像部(44)と、蛍光画像の輝度の観察距離における距離特性を累乗近似して得られる第1の指数の逆数で蛍光画像の輝度値を累乗して補正用蛍光画像を取得し、参照画像の輝度の観察距離における距離特性を累乗近似して得られる第2の指数の逆数で参照画像の輝度値を累乗して補正用参照画像を取得し、補正用蛍光画像を補正用参照画像により除算して補正蛍光画像(K)を取得する画像補正部(60)とを備える蛍光観察装置(101)を提供する。
