A physiological detection device includes a main body, a sensor, a signal processor and a calculation module. The sensor is disposed in the main body and the sensor is adapted to detect a detection portion of a human body so as to obtain a sensing signal. A signal processor is disposed in the main body, and the signal processor receives and processes the sensing signal, so as to output a digital physiological signal. The calculation module calculates and receives the digital physiological signal and obtains a first information and a second information of a plurality of features points of the digital physiological signal. The calculation module calculates the ratio of the second information and the first information, so as to obtain a physiological condition index. The digital physiological signal includes a plurality of waves generated according to a time sequence.一種生理檢測裝置包括本體、感測單元、訊號處理單元以及運算模組。感測單元配置於本體中,並且感測單元適於偵測人體的被測部位,以獲取感測訊號。訊號處理單元配置於本體中,且訊號處理單元接收感測訊號並對感測訊號進行處理,以輸出數位化生理訊號。運算模組接收數位化生理訊號,並計算獲取數位化生理訊號的多個特徵點的第一資訊及第二資訊。運算模組運算第二資訊與第一資訊的比值,以獲取生理狀態指數。數位化生理訊號包括依時序產生的多個脈波。數位化生理訊號的特徵點包括脈波的波峰點以及位於脈波的上升緣前端的起始點。100‧‧‧生理檢測裝置110‧‧‧本體120‧‧‧感測單元122‧‧‧光發射器124‧‧‧光接收器130‧‧‧訊號處理單元132‧‧‧濾波器134‧‧‧放大器136‧‧‧類比數位轉換器140‧‧‧運算模組142‧‧‧正規化處理單元144‧‧‧生理狀態指數運算單元150‧‧‧顯示單元180‧‧‧傳輸單元190‧‧‧記憶單元S1‧‧‧感測訊號S2‧‧‧數位化生理訊號