KOREA UNIVERSITY INDUSTRIAL & ACADEMIC COLLABORATION FOUNDATION
发明人:
HONG, KI CHANG,KIM, BYOUNG CHUL,PARK, SUNG SOO
申请号:
KR20060046014
公开号:
KR20070113336(A)
申请日:
2006.05.23
申请国别(地区):
韩国
年份:
2007
代理人:
摘要:
A DNA marker is provided to be used as a biological marker capable of increasing the meat amount as well as preventing the deterioration of the meat quality by an SNP portion in a 5'-promoter region of a Myogenin gene related to a pig muscle cell differentiation, thereby being useful for a pig breeding industry due to early selection and productivity improvement. A composition for detecting increase of pig muscle-cell number consists of a DNA fragment including a 788th T base in a sequence of SEQ ID : NO. 9. A primer for searching an SNP related to muscle-cell number of a 5'-promoter region of a Myogenin gene of a pig consists of oligonucleotides described as SEQ ID : NOs. 7 and 8. A kit for analyzing SNP related to the muscle cell number increase of a pig comprises the primer, a PCR reaction mixture and BspCNI, which is a restriction enzyme for analyzing RFLP. A method for identifying the muscle cell number increase of a pig comprises the steps of: (a) isolating a genomic DNA from the pig; (b) amplifying an