The effect of noise light originating in a light guide portion is removed by simple calculations, and a clear fluorescence image that facilitates distinction between lesion tissue and normal tissue is acquired. Provided is a fluorescence endoscope apparatus including an insertion portion inserted into a body cavity a light source unit that is disposed at a base end of the insertion portion and that emits excitation light and reference light that contains at least a part of the wavelength band of fluorescence produced by the excitation light a light guide portion that guides the excitation light and the reference light emitted from the light source unit to a distal end of the insertion portion an irradiation control unit that switches between a first irradiation state in which the excitation light guided by the light guide portion is radiated onto an inner wall of the body cavity and a second irradiation state in which the reference light is radiated onto the inner wall of the body cavity an image-acquisition unit that acquires reflected light of the reference light and the fluorescence returning from the inner wall of the body cavity to the insertion portion and an image computing unit that generates a fluorescence image signal by calculating the difference between a first image-acquisition signal acquired by the image-acquisition unit in the first irradiation state and a second image-acquisition signal acquired in the second irradiation state.
