KIM, BYUNG KIKR,김병기,OH, DONG YEPKR,오동엽,JUNG, DAE JINKR,정대진,YI, JUN KOOKR,이준구,HA, JAE JUNGKR,하재정,HWANG, EUN GYEONGKR,황은경
申请号：
KR1020150070346
公开号：
KR1020160137718A
申请日:
2015.05.20
申请国别(地区):
KR
年份:
2016
代理人:
摘要：
According to the present invention, a method for manufacturing medicinal herb probiotics for feed supplement comprises the following steps: 1) inoculating sterilized media with a starter to perform liquid cultivation in a shaking incubator at a temperature of 30 to 40°C and an agitation speed of 80 to 200 rpm for 20 to 28 hours 2) inoculating liquid media with the liquid cultivated starter to perform cultivation in a fermenter at a temperature of 30 to 40°C, an agitation speed of 50 to 150 rpm, and an oxygen utilization velocity of 0.5 to 1.5 vvm for 10 to 20 hours 3) inoculating solid media with the starter cultivated in the step 2) to perform solid cultivation at a temperature of 30 to 40°C and a humidity of 90% or more for 20 to 28 hours, wherein the solid media includes 60 to 85 wt% of wheat bran, 1 to 10 wt% of glucose, 1 to 10 wt% of sucrose, 1 to 10 wt% of peptone, 0.01 to 5 wt% of calcium hydrogen phosphate, 0.01 to 5 wt% of calcium carbonate, 1 to 10 wt% of an yeast extract, 1 to 5 wt% of minerals, and 1 to 5 wt% of growth factors 4) drying a solid cultivated body generated in the step 3) and mixing 0.01 to 5 wt% of the solid cultivated body dried in the step 4) with 30 to 60 wt% of a first medicinal herb product and 35 to 65 wt% of a yeast cultivated body. When the medicinal herb probiotics manufactured by the method of the present invention is fed to cattle by being added to feed, an effect of enhancing a fattening performance and improving meat quality is provided.COPYRIGHT KIPO 2016본 발명은 1) 멸균 배지에 종균을 접종하여 진탕배양기에서 온도 30~40℃, 교반속도 80~200rpm으로 20~28시간 동안 액체배양하는 단계 2) 액체 배지에 상기 액체배양한 종균을 접종하여 발효기에서 30~40℃, 교반속도 50~150rpm, 산소이용 통기량 0.5~1.5vvm으로 10~20시간 동안 배양하는 단계 3) 밀기울 60~85중량%, 포도당 1~10중량%, 수크로오스 1~10중량중량%, 펩톤 1~10중량%, 인산수소칼륨0.01~5중량%, 탄산칼슘 0.01~5중량%, 효모 추출물 1~10중량%, 미네랄 1~5중량% 및 성장인자 1~5중량%를 포함하는 고체배지에 상기 2) 단계에서 배양된 종균을 접종하고 온도 30~40℃, 습도 90% 이상으로 20~28시간 동안 고체배양하는 단계 4) 상기 3)단계로 생성된 고체배양체를 건조하는 단계 및 5) 상기 4) 단계의 건조된 고체배양체 0.01~5중량%을 1차 한방제조물 3
