Provided is a cell collection apparatus includes: a supporter that supports a sheet to which a back surface of a substrate is pasted, the substrate being divided in to plural small pieces with a section of a tissue pasted on a front surface thereof a needle including needle tips and a holder that is configured to hold the needle with the needle tips facing the sheet on an opposite side to a substrate side of the sheet, and to move the needle in the forward direction of the needle tips, wherein at least two needle tips are arranged within a region narrower than the back surface of the small piece substantially on the identical plane intersecting with the longitudinal direction of the needle.
