Image acquisition apparatuses, spectral apparatuses, methods and storage mediums for use with same are provided herein. At least one apparatus includes: a diffraction element irradiated by a light； a fiber for receiving reflected scattered light from a subject； a diffraction grating dispersing the transmitted light into light fluxes of wavelength bands again； an optical system imaging the split light fluxes； and an imaging device near the focal point of the optical system. An image is changed by rotating the diffraction grating, from which a two-dimensional image is acquired. The transmitted light may be branched into two or more, and input to a collimator lens and imaged as multiple spectral sequences by the optical system. At least one apparatus may form light fluxes traveling at different angles； and may acquire spectral information of the reflected and scattered light. Preferably, for the luminous fluxes, no gap exists in an image.
